anti ctr1  (Novus Biologicals)

Journal: Genes & Diseases

Article Title: SIRT5 mediates the pro-osteogenic effects of estrogen through FDX1 demalonylation and cuproptosis inhibition in mesenchymal stem cells

doi: 10.1016/j.gendis.2026.102145

Figure Lengend Snippet: Effects of ovariectomy or estradiol on SIRT5 expression and cuproptosis markers. (A) Micro-CT analysis results of the mice’s distal femur (scale bar: 1 mm). (B) Quantitative results of micro-CT analysis show the bone mineral density (BMD), the ratio of the bone volume to the total volume (BV/TV), the number of trabeculae (Tb.N), and the trabecular thickness (Tb.Th) ( n = 5). (C, D) Serum and bone tissue levels of Cu 2+ in mice ( n = 3). (E, F) Quantitative PCR and Western blotting assay results show the expression of FDX1, SIRT5, CTR1, and ATP7A. (G, H) Representative ICC images show the expression of FDX1 and SIRT5 in mice femur (scale bar = 50 μm for 1G or 25 μm for 1H). (I – L) Quantitative PCR assay results show the mRNA expression levels of Atp7a , Fdx1 , Sirt5 , and Ctr1 in C3H10T1/2 cells. (M) Western blotting assay results show the protein levels of FDX1, SIRT5, CTR1, and ATP7A in C3H10T1/2 cells treated with estradiol. (N) Quantitative results of Western blotting assay show the protein levels of SIRT5 in C3H10T1/2 cells treated with estradiol. (O) Western blotting assay results show the protein levels of SIRT5 in C3H10T1/2 cells treated with tamoxifen. (P) Quantitative results of Western blotting assay show the protein levels of SIRT5 in C3H10T1/2 cells treated with tamoxifen. ns P > 0.05, ∗P < 0.05, and ∗∗P < 0.01.

Article Snippet: Primary antibody against β-actin (AC026), RUNX2 (A2581), and FDX1 (A20895) were ordered from ABclonal (Wuhan, China); SIRT5 (5122-1-AP) and Ubiquitin (10201-2-AP) were ordered from Proteintech (Wuhan, China); Malonyllysine (PTM-901) was ordered from PTM Biolabs (Hongzhou, China); acetyl Lysine (ab80178) was ordered from Abcam (Shanghai, China); ATP7A (TD8506) and CTR1 (T510261F) were ordered from Abmart (Shanghai, China); OPN (sc-10593) was ordered from Santa Cruz Biotechnology (Shanghai, China).

Techniques: Expressing, Micro-CT, Real-time Polymerase Chain Reaction, Western Blot

Journal: Genes & Diseases

Article Title: SIRT5 mediates the pro-osteogenic effects of estrogen through FDX1 demalonylation and cuproptosis inhibition in mesenchymal stem cells

doi: 10.1016/j.gendis.2026.102145

Figure Lengend Snippet: Effects of copper or SIRT5 activator on osteoporosis in ovariectomized mice. (A) Micro-CT assay results of the distal femur of mice (scale bar = 1 mm). (B) Quantitative results of micro-CT assay show the bone mineral density (BMD), the ratio of the bone volume to the total volume (BV/TV), the number of trabeculae (Tb.N), and the trabecular thickness (Tb.Th) of femoral trabecular bone mass ( n = 5). (C) Representative images of hematoxylin-eosin and Masson’s trichrome staining show the effects of copper, tetrathiomolybdate (TTM, a copper chelator), and/or MC3138 (a SIRT5 activator) on ovariectomy (OVX)-induced bone loss (scale bar = 50 μm). (D, E) Representative images of immunofluorescent staining and quantitative results show the effect of copper and/or TTM on FDX1 affected by OVX. (F, G) Western blotting assay and quantitative results show the effects of copper or TTM on the protein level of FDX1, SIRT5, CTR1, and ATP7A affected by OVX. ns P > 0.05, ∗P < 0.05, and ∗∗P < 0.01.

Article Snippet: Primary antibody against β-actin (AC026), RUNX2 (A2581), and FDX1 (A20895) were ordered from ABclonal (Wuhan, China); SIRT5 (5122-1-AP) and Ubiquitin (10201-2-AP) were ordered from Proteintech (Wuhan, China); Malonyllysine (PTM-901) was ordered from PTM Biolabs (Hongzhou, China); acetyl Lysine (ab80178) was ordered from Abcam (Shanghai, China); ATP7A (TD8506) and CTR1 (T510261F) were ordered from Abmart (Shanghai, China); OPN (sc-10593) was ordered from Santa Cruz Biotechnology (Shanghai, China).

Techniques: Micro-CT, Staining, Western Blot

Journal: Genes & Diseases

Article Title: SIRT5 mediates the pro-osteogenic effects of estrogen through FDX1 demalonylation and cuproptosis inhibition in mesenchymal stem cells

doi: 10.1016/j.gendis.2026.102145

Figure Lengend Snippet: Effects of SIRT5 on the reduced osteogenic potential and cuproptosis induced by elesclomol (ES, 15 nM) in C3H10T1/2 cells. (A) Western blotting assay results show the levels of RUNX2 in cells treated with ES and/or AdSirt5. (B, C) ALP staining and quantitative results show the ALP activities in cells treated with ES and/or AdSirt5. (D) Western blotting assay results show the level of OPN in cells treated with ES and/or AdSirt5. (E, F) Alizarin red S staining and quantitative results show the mineralization levels in cells treated with ES and/or AdSirt5. (G) Representative transmission electron microscope images show the effect of ES and/or SIRT5 on mitochondria in C3H10T1/2 cells (scale bar = 500 nm). The red arrows indicate the damaged mitochondria, and the white arrows indicate the normal mitochondria. (H) Representative immunofluorescent staining images show the effect of ES and/or SIRT5 on mitochondria in C3H10T1/2 cells (scale bar = 10 μm). (I, J) JC-1 immunofluorescent assay and quantitative results show the effect of ES and/or SIRT5 on mitochondria in C3H10T1/2 cells (scale bar = 50 μm). (K) Copper content assay results show the effect of ES and/or SIRT5 on the medium or cellular level of Cu 2+ . (L, M) Western blotting assay and quantitative results show the effect of ES and/or SIRT5 on the level of FDX1, ATP7A, CTR1, and SIRT5 in C3H10T1/2 cells. ns P > 0.05, ∗P < 0.05, and ∗∗P < 0.01.

Article Snippet: Primary antibody against β-actin (AC026), RUNX2 (A2581), and FDX1 (A20895) were ordered from ABclonal (Wuhan, China); SIRT5 (5122-1-AP) and Ubiquitin (10201-2-AP) were ordered from Proteintech (Wuhan, China); Malonyllysine (PTM-901) was ordered from PTM Biolabs (Hongzhou, China); acetyl Lysine (ab80178) was ordered from Abcam (Shanghai, China); ATP7A (TD8506) and CTR1 (T510261F) were ordered from Abmart (Shanghai, China); OPN (sc-10593) was ordered from Santa Cruz Biotechnology (Shanghai, China).

Techniques: Western Blot, Staining, Transmission Assay, Microscopy

Journal: Parasites & Vectors

Article Title: Cuproptosis-driven astrocyte reactivity exacerbates experimental cerebral malaria pathogenesis

doi: 10.1186/s13071-025-07107-0

Figure Lengend Snippet: Effects of DSF or TTM treatment on astrocytic cuproptosis markers in ECM mice. A - E Representative immunohistochemical staining images of SLC31A1 (copper influx transporter), ATP7A (copper efflux transporter), FDX1 (key cuproptosis regulator), DLAT, and DLST (lipoylated precursor proteins) in the cerebral cortex captured under light microscopy (400 × magnification). Experimental groups: a naive mice; b DSF-treated uninfected controls; c TTM-treated uninfected controls; d untreated Pb A-infected mice; e DSF-treated infected mice; f TTM-treated infected mice. F Quantification of SLC31A1 + , ATP7A + , FDX1 + , DLAT + , and DLST + cells expressed as number per field. Data were obtained from > 20 fields per tissue slice, with six mice per group and three independent experimental replicates. Values represent mean ± standard deviation. Statistical significance: ** P < 0.01 and * P < 0.05 versus infected control (group d); && P < 0.01 versus naive mice (group a); NS (not significant, P > 0.05) indicates no difference compared with naive mice

Article Snippet: Sections were incubated with primary antibodies at 4 °C for overnight: GFAP (1:3,000; GB11096; Servicebio), Serping1 (1:1,000; GB112165 ; Servicebio), SLC31A1 (1:250; NB100-402; Novus), ATP7A (1:100; MA5-27720; Thermo Fisher), FDX1 (1:200; 12592–1-AP; Proteintech), DLAT (1:1,000; GB113649 ; Servicebio), DLST (1:1,500; GB114020 ; Servicebio), and S100B (1:500; GB15359; Servicebio).

Techniques: Immunohistochemical staining, Staining, Light Microscopy, Infection, Standard Deviation, Control

Journal: Parasites & Vectors

Article Title: Cuproptosis-driven astrocyte reactivity exacerbates experimental cerebral malaria pathogenesis

doi: 10.1186/s13071-025-07107-0

Figure Lengend Snippet: Effects of DSF or TTM treatment on GFAP + -SLC31A1 + and GFAP + -FDX1 + astrocytes in the cerebral cortex of ECM mice. A , B Representative double immunofluorescence staining images of GFAP + -SLC31A1 + and GFAP + -FDX1 + astrocytes in the cerebral cortex captured under fluorescence microscopy (400 × magnification). Experimental groups: a naive mice; b DSF-treated uninfected controls; c TTM-treated uninfected controls; d untreated Pb A-infected mice; e DSF-treated infected mice; f TTM-treated infected mice. GFAP + astrocytes (green fluorescence), SLC31A1 + or FDX1 + cells (red fluorescence), and co-localized GFAP + -SLC31A1 + or GFAP + -FDX1 + astrocytes (merged signal, yellow) are shown. C Quantification of GFAP + -SLC31A1 + and GFAP + -FDX1 + astrocytes expressed as number per field. Data were derived from > 20 fields per tissue slice, with six mice per group and three independent experimental replicates. Values represent mean ± standard deviation. Statistical significance: ** P < 0.01 and * P < 0.05 versus Pb A-infected control mice (group d); && P < 0.01 versus naive mice (group a); NS (not significant, P > 0.05) indicates no difference compared with naive mice

Article Snippet: Sections were incubated with primary antibodies at 4 °C for overnight: GFAP (1:3,000; GB11096; Servicebio), Serping1 (1:1,000; GB112165 ; Servicebio), SLC31A1 (1:250; NB100-402; Novus), ATP7A (1:100; MA5-27720; Thermo Fisher), FDX1 (1:200; 12592–1-AP; Proteintech), DLAT (1:1,000; GB113649 ; Servicebio), DLST (1:1,500; GB114020 ; Servicebio), and S100B (1:500; GB15359; Servicebio).

Techniques: Double Immunofluorescence Staining, Fluorescence, Microscopy, Infection, Derivative Assay, Standard Deviation, Control

Journal: Parasites & Vectors

Article Title: Cuproptosis-driven astrocyte reactivity exacerbates experimental cerebral malaria pathogenesis

doi: 10.1186/s13071-025-07107-0

Figure Lengend Snippet: Effects of DSF-CuCl 2 or TTM-CuCl 2 coculture on mRNA expression in iRBCs-stimulated astrocytes in vitro. Total RNA was isolated from astrocytes after coculture under the following conditions: a PBS-treated control (24 h or 48 h); b nonparasitized red blood cells (RBCs)-treated astrocytes (24 h or 48 h); c iRBCs-stimulated astrocytes (24 h or 48 h); d iRBCs-stimulated astrocytes cocultured with DSF-CuCl 2 (24 h or 48 h); e iRBCs-stimulated astrocytes cocultured with TTM-CuCl 2 (24 h or 48 h). mRNA levels of GFAP, Serping1, CXCL10, TNF-α, IL-1β, IL-6, SLC31A1, ATP7A, FDX1, DLAT, and DLST were quantified by qPCR using the 2 −ΔΔCT method. Data were derived from three independent experiments and expressed as mean ± standard deviation. Statistical significance: & P < 0.05 and && P < 0.01, or § P < 0.05 and §§ P < 0.01 versus PBS-treated astrocytes at 24 or 48 h, respectively; * P < 0.05 and ** P < 0.01, or # P < 0.05 and ## P < 0.01 versus iRBCs-stimulated astrocytes at 24 or 48 h, respectively; NS (not significant, P > 0.05) or ns (not significant, P > 0.05) indicates no difference compared with PBS-stimulated astrocytes at 24 or 48 h, respectively

Article Snippet: Sections were incubated with primary antibodies at 4 °C for overnight: GFAP (1:3,000; GB11096; Servicebio), Serping1 (1:1,000; GB112165 ; Servicebio), SLC31A1 (1:250; NB100-402; Novus), ATP7A (1:100; MA5-27720; Thermo Fisher), FDX1 (1:200; 12592–1-AP; Proteintech), DLAT (1:1,000; GB113649 ; Servicebio), DLST (1:1,500; GB114020 ; Servicebio), and S100B (1:500; GB15359; Servicebio).

Techniques: Expressing, In Vitro, Isolation, Control, Derivative Assay, Standard Deviation